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. 2020 Dec 10;14:601176. doi: 10.3389/fncel.2020.601176

Figure 1.

Figure 1

MSC-EVs reduce HI-induced striatal tissue injury. Histological brain injury was determined on cresyl violet stained 20 μm cryostat sections 7 days after HI, performed in 9-day-old C57BL/6 mice. Vehicle (0.9% NaCl, Veh), platelet-derived EVs (PL-EV), and MSC-EVs were administered i.p. 24, 72, and 120 h after HI. (A) Representative images of injured hemispheres for each experimental group are shown (scale bar: 1 mm). (B) Injury scores were assessed in different brain regions (cortex, hippocampus, and striatum) resulting in a sum score (total) quantified for each animal. (C) Brain atrophy was analyzed by measurement of intact areas on tissue sections at a distance of 400 μm between + 1 mm and – 2.6 mm from bregma. Volumes were calculated for total hemispheres and the indicated brain regions. Tissue loss was expressed as the percentage of volume reduction compared to intact contralateral volumes. *p < 0.05, **p < 0.01, n = 12 (Veh), n = 7 (PL-EV), n = 12 (MSC-EV).