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. 2020 Dec 10;14:601176. doi: 10.3389/fncel.2020.601176

Figure 3.

Figure 3

MSC-EVs increase regenerative proliferation. Cell proliferation was analyzed 7 days after HI in C57BL/6 mice exposed to HI on postnatal day 9. Vehicle (0.9% NaCl, Veh) and MSC-EVs were i.p. injected 24, 72, and 120 h after HI. The amount of proliferating cells was determined via immunohistochemistry for the proliferation marker Ki67 in the striatum and the subventricular zone (A,B). The percentage of proliferating endothelial cells (C) and oligodendrocytes (D) from all proliferating cells was quantified in tissue sections co-labeled for the endothelial cell marker CD31 and the oligodendrocyte marker Olig2, respectively. No co-labeling could be detected in co-staining of the neural precursor cell marker doublecortin and Ki67. Therefore, the absolute amount of DCX positive cells was quantified (E). *p < 0.05, **p < 0.01, n = 12/group, scale bar in (A): 500 μm (inset: 50 μm).