Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 21;12:1758835920974201. doi: 10.1177/1758835920974201

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s), 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 2. — (A) Clonogenic survival assay for olaparib in HeLa control and HeLa_XRCC1_KO cells untreated or treated with olaparib. (B) Quantification of γH2AX levels by flow cytometry in HeLa control and HeLa_XRCC1_KO cells treated with olaparib. (C) Quantification of cell cycle progression by flow cytometry in in HeLa control and HeLa_XRCC1_KO cells treated with olaparib. (D) Quantification of apoptotic cells by annexin V flow cytometry in in HeLa control and HeLa_ XRCC1_KO cells treated with olaparib. (E) Quantification of γH2AX levels by flow cytometry in HeLa control and HeLa_BRCA2_KO cells treated with olaparib. (F) Representative photo micrographic images of 231 control, 231 (XRCC1_KO), 157 cells, HeLa control and HeLa_XRCC1_KD 3D-spheroids treated with AZD6738 (5 μM) or treated with AZD6738 (5 μM) plus olaparib (5 μM). (G) Quantification of spheroid size in 231control, 231 (XRCC1_KO) and 157 treated with AZD6738 (5 μM) or treated with AZD6738 (5 μM) plus olaparib (5 μM). (H) Quantification of viable, dead cells by flow cytometry in 231control, 231 (XRCC1_KO) and 157 treated with AZD6738 (5 μM) or treated with AZD6738 (5 μM) plus olaparib (5 μM). (I) Representative photo micrographic images of HeLa control and (XRCC1_KO) cells treated with AZD6738 (5 μM) or treated with AZD6738 (5 μM) plus olaparib (5 μM). (J) Quantification of spheroid size in 231control, 231 (XRCC1_KO) and 157 treated with AZD6738 (5 μM) or treated with AZD6738 (5 μM) plus olaparib (5 μM). (K) Quantification of viable, dead cells by flow cytometry in 231control, 231 (XRCC1_KO) and 157 treated with AZD6738 (5 μM) or treated with AZD6738 (5 μM) plus olaparib (5 μM).

*p⩽ 0.05; **p⩽ 0.01; ***p-value ⩽ 0.001.