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. 2020 Dec 16;147(23):dev194449. doi: 10.1242/dev.194449

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — FIRE deletion differentially impairs microglial and BAM arrival dynamics. (A-C) Macrophage arrival dynamics in the (A) cephalic mesenchyme, (B) CNS parenchyma and (C) cerebroventricles of Csf1r^+/+, Csf1r^+/ΔFIRE and Csf1r^{ΔFIRE/ΔFIRE} embryos. Number of embryos per genotype (embryos per embryonic day): Csf1r^+/+, 8 (1-4); Csf1r^+/ΔFIRE, 16 (3-5); Csf1r^{ΔFIRE/ΔFIRE}, 17 (3-5). (D,E) Representative images of E12.5 embryonic heads from (D) Csf1r^+/ΔFIRE and (E) Csf1r^{ΔFIRE/ΔFIRE} mice stained with anti-IBA1 (red), anti-CD206 (green) and DAPI (blue). Auto-fluorescent erythrocytes are also yellow. Red lines depict the borders of the developing CNS tissue. Insets show (1) IBA1⁺CD206⁻ primitive microglia (pMG) and (2) IBA1⁺CD206⁺ BAMs. (F,G) Quantification of IBA1⁺CD206⁺ BAMs and IBA1⁺CD206⁻ primitive microglia in (F) cephalic mesenchyme and (G) CNS parenchyma of Csf1r^+/+, Csf1r^+/ΔFIRE and Csf1r^{ΔFIRE/ΔFIRE} embryos. Number of embryos per genotype (embryos per embryonic day): Csf1r^+/+, 10 (1-5); Csf1r^+/ΔFIRE, 16 (3-4); Csf1r^{ΔFIRE/ΔFIRE}, 17 (3-5). Mixed-effects analysis with Tukey-corrected multiple comparison post-tests were performed for A-C and F,G. Statistically significant differences compared with the Csf1r^+/ΔFIRE group are shown (*P<0.05, **P<0.01, ***P<0.001). Data are mean±s.e.m. Scale bars: 500 µm.