Fig. 2.

Immunohistochemical localization of merCremer in cardiomyocytes (CMs). Hearts from mice treated with tamoxifen, as described in Fig. 1, were histologically processed and double-immunostained using antibodies to detect the presence of merCremer in the nucleus, and, to verify CM identity, cardiac troponin T (cTnT) in the cytoplasm. In each heart, a minimum of 1000 CMs were evaluated for nuclear merCremer by examining 200× microscopic images from six randomly selected areas. Arrows point to examples of merCremer-positive CMs.