Fig. 4.

qRT-PCR determinations showing activation of genes that regulate cell-cycle stage G₂/M, and the gene encoding p21, in hearts following tamoxifen-induced activation of merCremer. (A-C) The effect of tamoxifen-activated merCremer on the expression of genes in the heart that activate cell-cycle stages G₂/M (A) and G₁ (B), and genes that inhibit the cell cycle (C). The expression of each gene was normalized to Gapdh. In each panel, colored lines denote expression of each gene in +/+;Myh6-merCremer hearts, relative to expression in +/+ wild-type controls at y=1 (dashed lines). n=3 hearts per time point; *P<0.05 versus +/+.