Fig. 6.

Auxin homeostasis regulates root thermomorphogenesis. (A-C) Normalized root growth (27°C/21°C) in wild-type (WT), tir1, afb2, tir1 afb2 (A), tmk1,4 (B) and yucQ (C) seedlings. (D) Differentially regulated genes in hy5 and phyAB roots at 27°C that are auxin (IAA) responsive according to Omelyanchuk et al. (2017), 4 h after temperature shift. (E) IAA concentration [pmol/g of fresh weight (FW)] in roots of seedlings at 6 DAG, 12 h after transfer to 21°C or 27°C (n>3). (F) Relative IAA content in roots compared with those in shoot tissues of seedlings 6 DAG, 12 h after transfer to 21°C or 27°C (n>3). n indicates the number of individual seedlings measured (A-C) or the number of biological replicates (E,F). Measured seedlings were obtained in three (A-C) independent replications of the experiment. In A,E,F, groups denoted by different letters are significantly different from one another [P<0.05; one-way ANOVA and Tukey HSD post hoc test (A,E) or Student–Newmann–Keuls post hoc test (F)]. Other P-values were calculated using a two-tailed, unpaired Student's t-test (B,C) or hypergeometric test (D). Red bar represents the mean (A-C).