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. 2020 Dec 13;147(23):dev194274. doi: 10.1242/dev.194274

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Constitutive expression of SPL/NZZ induced the formation of multiple MMC-like cells. (A-E) DIC microscopy (A-C) and confocal microscopy (D,E) of 35S::SPL/NZZ lines. Multiple enlarged cells can be observed in the premeiotic ovule nucellus, highlighted with different colours. Confocal sections were acquired after Feulgen staining. Scale bar: 15 µm in A (for A-C). (F) Graphical representation of the percentage of multiple enlarged cells in eight 35S::SPL/NZZ independent lines. ***P<0.001 by Student's unpaired t-test. Wild-type premeiotic ovules were screened as the control.