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. 2020 Dec 18;13(12):dmm045377. doi: 10.1242/dmm.045377

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Cardiac-specific knockdown of CD14964 leads to dosage-dependent heart defects in the adult fly. (A-D). End-diastolic diameter (EDD) of 3-week-old flies harboring a knockdown of CG14964 by using (A) CG14964-GDi crossed with Hand⁴^.2-Gal4, (B) CG14964-GDi crossed with Mef2-Gal4, (C) CG14964 deficiency or (D) transheterozygous CG14964^CRIMIC/deficiency. We observed heart dilation (A-C, mild knockdown) or constriction (D, strong knockdown). All raw data are shown (mean±s.d.), as well as effect size and 95% c.i. below the data. In addition, Phalloidin-stained cardiac myofibrils show altered heart diameters in representative examples (measurements taken at the green lines). Scale bars: 50 μm.