Figure 1.

miRNAs Are Essential for Morphogenesis and Organogenesis

(A) Schematic of the genetic setup to deplete the Microprocessor via the AID system and hatching rate of control (expressing only TIR1) or Microprocessor-depleted embryos by RNAiD. n, number of embryos scored; N, number of independent experiments. Error bars represent the standard error of the proportion.

(B) Absolute miRNA abundance in WT and RNAiD embryos at the 2-cell stage and at the end of gastrulation as determined by quantitative small RNA sequencing. The 100 most abundant miRNAs in WT embryos at each stage are shown. Remaining miRNAs of the miR-35 and miR-51 families are depicted in gray. The percentage of miRNA molecules remaining relative to WT is indicated, with relative contributions of miR-35 and miR-51 families combined noted in brackets. Note that the samples analyzed here are the same as in Figures S2F, S2G, 4A, and S4A.

(C) Schematic of the predominant time of arrest and representative images of embryos of the indicated genotypes.

(D) Absolute miRNA abundance in developing WT embryos as measured by small RNA-seq, highlighting the proportion of miR-35 and miR-51 families.

See also Figure S1.