Figure 4.

Mirtron-Rescued Embryos Lack Canonical miRNAs at the Molecular and Functional Level

(A) Absolute miRNA abundance in WT, RNAiD, and pash-1(ts) animals under restrictive conditions, at the indicated time points, measured by spike-in normalized small RNA-seq as in Figure 1B. Shown are the top 100 miRNAs, ranked by abundance in WT samples. Remaining miRNAs of the miR-35 and -51 families are in gray. For comparison, data for 2-cell and gastrulation time points are replotted from Figures 1B and S1G. The percentage of miRNA molecules remaining relative to WT is indicated, with the relative contribution of mir-35 and mir-51 families combined noted in brackets. For quantification of miRNAs grouped by families, see Figure S4A.

(B) Functional readout for the function of the late embryonic miRNA lsy-6. In WT animals, only the right ASE neuron expresses gcy-5:gfp, but absence of functional lsy-6 results in activation of the reporter in both the left and right neurons.