Figure 2.

BASL Is Polarized in Protoplasts

(A) Light field view of protoplast after 3 h in protoplasting solution.

(B) Distribution of GFP-BASL in the same protoplast as (A).

(C) Formation of protoplast. First time point (0 min) taken a few minutes after addition of protoplasting solution. Nuclear division between 80 min and 140 min (no cell wall forms as the cells are in protoplasting solution). Magenta = cell wall stained with Direct Fast Red B.

(D) Polarized BASL is dynamic and not coordinated in protoplasts. Z-slices taken from the top (left column) or middle (right column) plane of protoplasts at different times. In protoplast (a), polarized BASL appears on the side face of (middle, 150 min). In protoplast (b), polarized BASL disappears from the side face (middle, 150 min) and reappears in a new location (middle, 600 min). In protoplast (c), polarized BASL moves from side to top face (top, 270 min). In protoplast (d), a transition with two foci of polarized BASL occurs. The side face locations of polarized BASL changes after cell division in protoplasts (b) and (d). Arrows indicates polarized BASL; N, nucleus.

Scale bars (A–C): 15 μm; (D): 20 μm. Time (C) and (D) = min. See also Videos S1 and S2; Figure S4.