Figure 5.

Variations in Cortical State Are Not Fully Explained by Variations in Pupil Size

(A) Probability of Miss trials at increasing pupil sizes.

(B) Relationship between pupil size, behavioral condition, and 3- to 6-Hz power in somatosensory cortex of an example experiment. Each dot represents a trial, colored according to outcome: Choice (green) or Miss (dark gray). The lines represent fits from an ANCOVA model, which captured the main effect of behavioral condition as the difference in intercept between the Choice and Miss fits (blue arrow).

(C and D) Similar analysis for visual and motor cortex ROIs.

(E) Pseudocolor maps showing intercept difference for each pixel, for the same example sessions shown in previous figures. Blue indicates significantly higher power prior to Miss trials, after accounting for the common effect of pupil size.

(F) Summary of intercept differences across experiments in selected ROIs (n = 48 experiments from 12 animals).

(G) Same analysis as in (F) but with the temporal derivative of pupil size.

See also Figure S7.