Figure 2.

Disrupted Aldehyde Catabolism Compromises Hematopoiesis

(A and B) Representative flow cytometry plots from Aldh2^−/−Adh5^−/− and WT mice showing bone marrow LK, LKS, LT-HSC, CLP, and CMP (A) and myeloid populations (B). Bottom: quantification of the respective populations assessed by flow cytometry in 2- to 30-week-old Aldh2^−/−Adh5^−/− mice with age-matched controls (mean ± SEM; n = 24, 20, 17, 17, left to right).

(C) scRNA-seq analysis of HSPCs from a 6-week-old female Aldh2^−/−Adh5^−/− mouse with age- and sex-matched controls.

(D) Fraction of single-cell transcriptomes assigned to the HSC cell identity (numerator) from total transcriptomes analyzed (denominator).

(E) hscScore analysis of single-cell transcriptomes identified as HSCs.

(F) UMAP visualization of HSC transcriptomes colored by cluster. On the left, all 4 genotypes are superimposed; on the right, individual genotypes are shown separately to highlight variation in distribution between the clusters.

The p values were determined by two-tailed Mann-Whitney U test. See also Figures S2 and S3 and Tables S2 and S5.