Fig. 4.

Densitometry analysis of levels of filament-associated flagellin. (A) SDS-PAGE gels for each biological replicate, from which densitometry measurements were taken. Flagella were depolymerised by heat treatment and the monomers were separated from cells and denatured before loading onto gels with their quantities normalised by cell density. S = molecular weight standards; L = low copy number (RK2) plasmid; M = medium copy number (p15a) plasmid; H = high copy number (pUC) plasmid. (B) Log2-transformed levels of filament-associated flagellin in each strain relative to wild-type AW405. A fliC knockout strain gave a small signal by densitometry that was not distinguishable from background noise and other proteins, and so is not shown here. Results are the means of 3 biological replicates ±1 standard deviation.