Table 1.
Bacterial strains and plasmids used in this study.
| Name | Genotype/Features | Antibiotics | Source/ref. |
|---|---|---|---|
| Bacterial strains | |||
| DH5α | F−, Φ80lacZΔM15, Δ(lacZYA-argF), U169, recA, endA1, hsdR17(rk−,mk+), phoA, supE44, thi-1, gyrA96, relA1, λ− | None | Invitrogen cat. No. 18265017 |
| AW405 | WT for motility and chemotaxis F−, thr1, araC14, leuB6, fhuA31, lacY1, tsx-78, glnX44, galK2, galT22, λ, hisG4, rfbC1, mgl-51, rpsL136, xylA5, mtl-1, thiE1 |
Streptomycin | (Turner et al., 2012) |
| AW405ΔfliC | As for AW405 but with fliC deleted; motility− | Streptomycin | This study |
| Plasmids | |||
| pDOC-K | Donor plasmid for gene doctoring, with sacB gene for counterselection of successful transformants on sucrose medium | Kanamycin, Carbenicillin | (Lee et al., 2009) |
| pACBSCE | Helper plasmid for gene doctoring | Chloramphenicol | (Lee et al., 2009) |
| pCP20 | Temperature-sensitive helper plasmid for kanR removal by FLP recombinase | Carbenicillin | (Lee et al., 2009) |
| pBb(RK2)1 k-GFPuv | PCR template for RK2 replication origin | Kanamycin | Addgene #106394, (Cook et al., 2018) |
| pJ211_WT_med | Expression plasmid for FliC fliC promoter; p15a (medium copy-number) replication origin |
Kanamycin | ATUM custom order |
| pJ291_WT_med | Expression plasmid for FliC T5 promoter; p15a replication origin |
Kanamycin | ATUM custom order |
| pD441_WT_hi | Expression plasmid for FliC T5 promoter; pUC (high copy-number) replication origin |
Kanamycin | ATUM custom order |
| pJ211_WT_low | pJ211_WT_med derivative fliC promoter; RK2 (low copy-number) replication origin |
Kanamycin | This study |
| pJ211_WT_hi | pJ211_WT_med derivative fliC promoter; pUC origin |
Kanamycin | This study |
| pJ211_rha_WT_low | pJ211_WT_med derivative rha promoter; RK2 origin |
Kanamycin | This study |
| pJ211_rha_WT_med | pJ211_WT_med derivative rha promoter; p15a origin |
Kanamycin | This study |
| pJ211_rha_WT_hi | pJ211_WT_med derivative rha promoter; pUC origin |
Kanamycin | This study |
| pJ291_WT_low | pJ291_WT_med derivative T5 promoter; RK2 origin |
Kanamycin | This study |