Figure 3.
Crosslinking Mass Spectrometry Analysis of Smc5/6 Complex Architecture
(A) SDS-PAGE analysis of the Smc5/6 complex before and after BS3 treatment.
(B) Intra- and inter-subunit crosslinks of the Smc5/6 complex.
(C) Inter-subunit crosslinks between Smc5 and Smc6 proteins. Head and hinge regions were annotated accordingly to Duan et al. (2009b). Throughout the whole figure, crosslinks indicating an interaction with the head and hinge regions of the Smc subunits are highlighted in blue.
(D) Smc6 intra-subunit crosslinks.
(E) Inter-subunit crosslinks of the non-SMC elements.
(F) Tentative topology of the Smc5/6 complex based on the crosslinking mass spectrometry data.
See Table S2 for further characterization of the Smc5/6 crosslinking mass spectrometry analysis.