REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-HA (12CA5)	Sigma-Aldrich	Sigma-Aldrich Cat# 11583816001, RRID:AB_514505
Anti-Smt3	Abcam	Abcam Cat# ab14405, RRID:AB_301186
Anti-rabbit IgG (HRP-conjugated)	Thermo Fisher Scientific	Thermo Fisher Scientific Cat# 10710965, RRID:AB_772191
Anti-mouse IgG (HRP-conjugated)	GE Healthcare	GE Healthcare Cat# NA931, RRID:AB_772210
Bacterial and Virus Strains
Stbl2 E.coli	Invitrogen	Cat#10268019
Rosetta 2 (DE3) pLysS E.coli	Sigma-Aldrich	Cat#CMC0014
Chemicals, Peptides, and Recombinant Proteins
DTT	Sigma-Aldrich	Cat#43815-5G
ATP	Sigma-Aldrich	Cat#A2383
biotin	Sigma-Aldrich	Cat#B4501-1G
cOmplete EDTA-Free protease inhibitor cocktail	Sigma-Aldrich	Cat#11873580001
BSA	Thermo Fisher	Cat#AM2616
Benzonase	Sigma-Aldrich	Cat#1000.01695.0001
Desthiobiotin	Sigma-Aldrich	Cat#D1411-1G
InstantBlue	Sigma-Aldrich	Cat#IBSL-1L
NuPAGE 4-12% Protein gels	Thermo-Fisher	Cat#NP0321PK2
Amicon Ultra	Sigma-Aldrich	Cat#UFC5003
Phospho(enol)pyruvic acid	Sigma-Aldrich	Cat#P7127
Pyruvate kinase	Sigma-Aldrich	Cat#P9136
Lactate dehydrogenase	Sigma-Aldrich	Cat#SAE0049
NADH	Sigma-Aldrich	Cat#10107735001
β-mercaptoethanol	Sigma-Aldrich	Cat#M6250
Imidazole	Sigma-Aldrich	Cat#I2399
Oriole Gel stain	BioRad	Cat#161-0495
SYBR Safe gel stain	Thermofisher	Cat#S33102
Critical Commercial Assays
StrepTrap HP	Cytivia	Cat#28907548
HiTrap Heparin HP 5ml	Cytivia	Cat#GE17-0407-01
Superose 6 10/300 GL	GE Healthcare	Cat#17517201
Ni-NTA Superflow	QIAGEN	Cat#30410
Dynabeads MyOne Streptavidin	ThermoFisher	Cat#65601
Deposited Data
Protein-protein crosslink mass spectrometry (CLMS) data	PRIDE	PXD016196
Experimental Models: Organisms/Strains
Yeast strain: Mata Lys2::pGAL1-GAL4::LYS2 pep4::HIS3 bar1::hisG ade2-1 trp1D2 can1-100 leu 2-3,112	Aragon lab	n/a
Yeast strain: CCG14584 -Mata Lys2::pGAL1-GAL4::LYS2 pep4::HIS3 bar1::hisG ade2-1 trp1D2 can1-100 leu 2-3,112 [pRS424-GAL-SMC6-3xStrepII-SMC5-NSE4-His-3HA] [pRS426-GAL-NSE1-NSE3-NSE6-NSE2-NSE5]	Aragon lab	n/a
Yeast strain: CCG14619 -Mata Lys2::pGAL1-GAL4::LYS2 pep4::HIS3 bar1::hisG ade2-1 trp1D2 can1-100 leu 2-3,112 [pRS424-GAL-SMC6-3xStrepII-SMC5-NSE4-His-3HA] [pRS426-GAL-NSE1-NSE3-NSE6-NSE2-NSE5].	Aragon lab	n/a
Oligonucleotides
Magnetic tweezers oligo 1 5′GCGTAAGTGGTACCTT ATAAAGTACTCGACTCAC TATAGGGAGACCGGC-3′	Sigma-Aldrich	n/a
Magnetic tweezers oligo 2 5′-AGTAAGCGCCGTCAGACCAG-3′	Sigma-Aldrich	n/a
Recombinant DNA
Plasmid- CCG1198 [pRS424-GAL1-SMC6-3xStrepII-SMC5-NSE4-His-3HA TRP1]	Aragon lab	n/a
Plasmid- CCG1204 [pRS426-GAL1-NSE1-NSE3-NSE6-NSE2-NSE5 URA3]	Aragon lab	n/a
Plasmid- CCG1267 [pRS426-GAL-SMC4StrepII-SMC2-BRN1-His6-3HA URA3]	Haering lab	Terakawa et al. 2017
Plasmid- CCG1268 [pRS424-GAL-YCS4-YCG1 TRP1]	Haering lab	Terakawa et al. 2017
Software and Algorithms
xiVIEV	Rappsilber lab	N/A
MaxQuant	Max Planck, Martinsried, Germany	https://maxquant.org
OriginPro 8	Originlab Corporation	https://www.originlab.com/
LabVIEW software	Moreno-Herrero lab	N/A
RELION	Scheres lab	https://www2.mrc-lmb.cam.ac.uk/groups/scheres/impact.html
Image Lab	BioRad	Cat#17006130
Fiji ImageJ	Open source	https://imageJ.net/Fiji
Other
Negatively supercoiled pBR322	Inspiralis	Cat#S5001
Positively supercoiled pBR322	Inspiralis	Cat#POS5001
Catenated DNA (kDNA)	Inspiralis	Cat#K2001