Table 1. CSF Comparison of Normotensive Pregnant and Nonpregnant Women Analyzed with the Progenesis Methoda.
| protein name | gene name | up/down regulated in CO | fold change (CO/NP) | pregnancy hits | total literature hits | ratio pregnancy/total hits | weighted score | reference of first publication |
|---|---|---|---|---|---|---|---|---|
| adrenomedullin | ADML | up | 2.04 | 139 | 3517 | 0.040 | 5.49 | 10.1016/S0002-9378(96)80023-8 |
| thioredoxin | TXN | up | 1.92 | 68 | 11 873 | 0.006 | 0.39 | PubMed ID: 1700994 |
| growth arrest-specific protein 6 | GAS6 | up | 2.50 | 15 | 739 | 0.020 | 0.30 | 10.1152/ajplung.1998.275.6.L1184 |
| lumican | LUM | up | 1.69 | 10 | 646 | 0.015 | 0.15 | 10.1016/S0945-053X(98)90122-6 |
| γ-enolase | ENO2 | up | 1.43 | 3 | 148 | 0.020 | 0.06 | 10.1530/REP-08-0469 |
| selenoprotein P | SEPP1 | down | 0.58 | 3 | 192 | 0.016 | 0.05 | 10.1017/S0007114514000531 |
| profilin-1 | PFN1 | up | 2.33 | 3 | 258 | 0.012 | 0.03 | 10.1371/journal.pone.0031418 |
| transaldolase | TALDO1 | up | 2.33 | 1 | 36 | 0.028 | 0.03 | 10.1016/j.jpeds.2006.08.016 |
| complement factor D | CFD | down | 0.41 | 2 | 156 | 0.013 | 0.03 | 10.1371/journal.pone.0125346 |
| neuropilin-1 | NRP1 | down | 0.75 | 4 | 692 | 0.006 | 0.02 | 10.1002/(SICI)1526-968X(200003)26:3 < 213::AID-GENE7 > 3.0.CO;2-M |
| receptor-type tyrosine-protein phosphatase N2 | PTPRN2 | up | 2.27 | 1 | 56 | 0.018 | 0.02 | 10.1097/QAD.0000000000001124 |
| ATP-binding cassette subfamily A member 2 | ABCA2 | up | 1.89 | 1 | 92 | 0.011 | 0.01 | 10.1074/jbc.M611056200 |
The comparison of normotensive women with nonpregnant women; 574 peptides with p < 0.01 corresponding to 23 significant differential proteins were identified with the Progenesis method. The number of background peptides was 66 ± 27, calculated by a permutation test. Twenty of the 23 proteins are shown to be upregulated in normotensive pregnant women (see Table S2, Supporting Information). Twelve (out of 23) proteins were described in the literature as being associated with pregnancy listed above. Each protein was ranked to its weighted score in the Scopus citation database search related to pregnancy. In the last column, the first published paper is listed that described the protein as pregnancy-associated. Proteins (n = 11) with zero counts (no association with pregnancy) for the weighted score are shown in Table S2. CO = normotensive pregnant women; NP = nonpregnant women.