Table 1.
Primary antibodies. IF: immunofluorescence, WB: Western blot, IHC: immunohistochemistry
| Antibody | Clone | Species | Company | Concentration |
|---|---|---|---|---|
| p44/42 MAPK (Erk1/2) | 137F5 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) |
D13.14.4E XP® |
Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
| Src | 36D10 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
| Phospho-Src (Ser17) | D7F2Q | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
| AMPKα | D5A2 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
| Phospho-AMPKα (Thr172) | 40H9 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
| p38α MAPK | 7D6 | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
| Phospho-p38 MAPK (Thr180/Tyr182) | D3F9 XP® | Rabbit | Cell Signaling, Leiden, The Netherlands | 1:1000 |
| Anti-Acetylcholine-esterase (AChE) | Rabbit pAb | Prestige Antibodies®, Sigma-Aldrich, Taufkirchen. Germany |
1:200 (IF) 1:1000 (WB) 1:400 (IHC) |
|
| Anti-alpha-Tubulin | Ab11034 | Rabbit | Abcam, Cambridge, UK | 1:10.000 |
| F4/80 | Ab6640 | Rat | Abcam, Cambridge, UK | 1:75 |
| CD45 | Ab10558 | Rabbit | Abcam, Cambridge, UK | 1:100 |
| ChAT | Polyclonal | Rabbit | Kindly provided by Prof. M. Schemann, TU Munich | 1:1000 |