Figure 1.

dSTORM imaging of Trop2 on apical and basal membranes of A549 and HBE cells. (a–d) Reconstructed dSTORM images of Trop2 on apical (a) and basal (c) membranes of A549 cells. The magnified images are shown in (b,d). (e–h) Reconstructed dSTORM images of Trop2 on apical (e) and basal (g) membranes of HBE cells. The magnified images are shown in (f,h). Scale bars: 5 μm in original images and 1 μm in magnified images. (i) Number of Trop2 localizations per μm² on apical and basal membranes of both kinds of cells. (j) Number of clusters per μm². (k) Cluster area. (l,m) Percentage of clusters containing different number of localizations on (l) A549 cells and (m) HBE cells. All results were obtained from 10 cell samples in 5 independent experiments. Statistical significance was processed by two-tailed unpaired t-test. *P < 0.05, **P < 0.01, ***P < 0.001, and ns means not significant.