Figure 4.

CB treatment weakened Trop2 clustering on apical membranes. (a–d) Reconstructed dSTORM images of Trop2 on control (a) and CB-treated (c) A549 cell membranes and the corresponding magnified images (b,d). Scale bars: 5 μm in (a,c) and 1 μm in (b,d). (e) Number of Trop2 localizations per μm² on control and CB-treated A549 cell membrane. (f) Number of clusters per μm². (g) Cluster area. (h) Percentage of clusters containing different number of localizations. All results were obtained from 10 cell samples in 5 independent experiments. Statistical significance was processed by two-tailed unpaired t-test. *P < 0.05, **P < 0.01, ***P < 0.001, and ns means not significant.