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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Cancer Res. 2020 Jun 9;80(15):3130–3144. doi: 10.1158/0008-5472.CAN-19-3105

Figure 2. Lymphatic Cellular Environment Proficiently Induces Lytic Replication.

Figure 2.

(A,B) Primary BECs and LECs were infected with different amounts of KSHV RG to achieve a similar initial infectivity (~20%) at day 1 post-infection. The cells were then continuously cultured for two weeks in virus-free media before flow cytometry assays to determine the percentage of infected cells (A) and the ratio of lytic cells (green) over infected (red) cells (B). (C-F) Primary BECs and LECs were seeded at a different density (5, 10, 25, 50 and 100%) and then infected with KSHV RG to ~30% of LECs (C,D) or 45% of BECs (E,F). Percent of infected cells (C,E) and the ratio of lytic cells over infected cells (D,F) were measured over two weeks. (G-K) At 6, 12, and 24 hours post-infection, total viral genome copy number (G) and the expression of KSHV lytic genes (H-K) were determined by real-time quantitative PCR or RT-PCR assays, respectively. More than five sets of same donor-derived LECs and BECs were used, and the similar results were obtained. Statistics, 2-tailed t-test, *, p <0.05; **, p <0.01; ***, p <0.001.