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. Author manuscript; available in PMC: 2020 Dec 24.
Published in final edited form as: Clin Chem. 2018 Apr 23;64(7):1085–1095. doi: 10.1373/clinchem.2017.285072

Fig. 1. Comparison of RNA isolation efficiency with commercial kits and their performance on human CFS samples.

Fig. 1.

Total RNA yield from different kits was measured with Ribogreen RNA assay (A).The recovery of mRNAs of 3 reference genes (GAPDH, ACTB, and RPS9) from different kits was determined with RT-qPCR (B). The Ct valures of each gene from different kits were plotted. Recovery of miRNAs (miR148a, miR21, and miR26a) (C) and piRNAs (piR-001184, piR-014923, and piR-01857) (D) of each isolation kit was measured with droplet digital PCR(ddPCR) method. The relative yield of each small RNA was normalized by setting the highest mean copy number from 1 kit as 100%.