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. Author manuscript; available in PMC: 2020 Dec 24.
Published in final edited form as: Clin Chem. 2018 Apr 23;64(7):1085–1095. doi: 10.1373/clinchem.2017.285072

Fig. 3. Optimized rRNA depletion can increase the sensitivity of human gene detection.

Fig. 3.

Relative RNA yield after rRNA depletion measured with the Quant-iT RiboGreen RNA Assay Kit (Invitrogen) (A). The relative yield of 16S rRNA was examined with RT-qPCR (B). The relative yield of human reference RNA was measured with RT-qPCR (C). Comparison of gene coverage with different cutoff RPKM thresholds (D). Comparison of detected genes in rRNA depleted and non-depleted CFS samples for long and for small libraries (E).