Table 1.
Comparison of different RNA extraction kits.
| RNA isolation kit | miRNeasy Micro Kit | TRIzol Plus RNA Purification Kit | Quick-RNA MicroPrep kit | QIAamp Viral RNA Mini Kit | NucleoSpin miRNA kit | mirVana miRNA Isolation Kit |
|---|---|---|---|---|---|---|
| Principle | Phenol/guanidine-based and silica membrane-based method | GITC-based method | Nonphenol-based, Zymo-Spin column technology | Nonphenol-based, silica membrane-based spin column or vacuum technology | Nonphenol-based, silica membrane-based mini spin column technology | Phenol-based, organic extraction with spin column technology (Glass Fiber Filter) |
| Combined method of organic extraction and spin filter clean up | Organic extraction method | Combined method of organic extraction and spin filter clean up | Spin filter-based method | Spin filter-based method | Combined method of organic extraction and spin filter-based method | |
| Size of RNAs | Total RNA, including miRNA and other small RNAs (smaller than ~200 nucleotides) | Total RNA | Total RNA (including small RNAs 17–200 nucleotides) | Total viral RNA greater than 200 nucleotides | Total RNA including miRNA, siRNA, shRNAa (small RNA: <200 nucleotides, large RNA: >200 nucleotides) | Total RNA including miRNA, siRNA, shRNA, and snRNA |
| Sample source | Cultured cells, various animal and human tissues | Animal and plant cells, tissue, bacteria, or yeast | Any cells (animal, blood cells, etc.), all tissues, yeast, plant, or bacteria | Plasma (treated not with heparin), serum, and other cell-free body fluids | Cultured cells, human/animal tissue, plant tissue, <150 µL reaction mixture | All cell and tissue types, bacteria, plants, viral samples, and yeast |
a
shRNA, short (or small) hairpin RNA.