Skip to main content
. 2020 Dec 7;9:e60047. doi: 10.7554/eLife.60047

Figure 8. Unlike organelles, artificial cargoes functionalized with dynein move at constant speed throughout asters.

Figure 8.

(A) Artificial cargoes were functionalized with an antibody against the dynein adapter HOOK2, and negative control beads were functionalized with random antibody (see Video 12). (B) Max intensity projections of beads functionalized with anti-HOOK2 (red) or random antibody (green). (C) Trajectories of anti-HOOK2 and negative control beads relative to the MTOC. The growing aster is indicated by the blue region. Anti-HOOK2 beads started to be transported when they were engulfed by the growing aster. (D) Velocity distribution of anti-HOOK2 beads inside the aster. (E–H) Similar experiment with F-actin fragmented by Cytochalasin D.

Figure 8—source data 1. Bead trajectories for panels C, D, G, and H.
The ‘beads’ variables are structures that contain the bead trajectories in XY, as well as the distance from the MTOC at the aster center. The ‘TinsideAster’ variables are the time index at which beads first enter the aster, which was used to generate the velocity distributions in panels D and H.