Video 4. Signatures of both co-movement and relative movement in moving asters imaged at 60x.

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(Related to Figure 3—figure supplement 1) MTs were labeled with tubulin-Alexa Fluor 647, ER with DiI, and F-actin with Lifeact-GFP. Imaged on a spinning disk confocal with 60x objective lens. All networks were highly dynamic. Some ER and F-actin features moved relative to astral MTs, deformed, or otherwise changed structure, which provide examples where co-movement breaks down on small spatiotemporal scales; however, the dominant trend was co-movement of the networks.