Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-FXFG repeats (mouse monoclonal) | Abcam | Cat# ab24609 | IF (1:1000) |
| Antibody | Anti-FLAG tag (mouse monoclonal) | Sigma | Cat# F3165 | IF (1:1000) |
| Antibody | Anti-IRF3 (rabbit polyclonal) |
Santa Cruz biotechnology | Cat# sc-9082 | IF (1:400) |
| Antibody | Anti-rabbit alexa fluor 488 IgG (goat polyclonal) | Invitrogen | Cat# A-11008 | IF (1:500) |
| Antibody | Anti-mouse Alexa Fluor 546 IgG (goat polyclonal) | Invitrogen | Cat# A-11030 | IF (1:500) |
| Antibody | Anti-VSV-G (rabbit polyclonal) | Sigma | Cat# V4888 | WB (1:20,000) |
| Antibody | Anti-HIV-1 p24 (mouse monoclonal) | NIH AIDS reagent program | Cat# 3537 | WB (1:1000) |
| Antibody | Anti-STING (Rabbit monoclonal) | Cell Signaling | Cat# 13647 | WB (1:1000) |
| Antibody | Anti-phospho STING (Rabbit monoclonal) | Cell Signaling | Cat# 19781 | WB (1:1000) |
| Antibody | Anti-TBK1 (Rabbit monoclonal) | Cell Signaling | Cat# 3504S | WB (1:1000) |
| Antibody | Anti-phospho TBK1 (Rabbit monoclonal) | Cell Signaling | Cat# 5483 | WB (1:1000) |
| Antibody | Anti-IRF3 (Rabbit monoclonal) | Cell Signaling | Cat# 4302 | WB (1:1000) |
| Antibody | Anti-phospho -IRF3 S386 (Rabbit monoclonal) | Abcam | Cat# ab76493 | WB (1:1000) |
| Antibody | Anti-phospho- IRF3 S396 (Rabbit monoclonal) | Cell signaling | Cat# D6O1M | Flow Cytometry (1:50) |
| Antibody | Anti-actin (mouse polyclonal) | Abcam | Cat# ab8227 | WB (1:20,000) |
| Antibody | Anti-cGAS (rabbit monoclonal) | Cell Signaling Technology | Cat# 15102 | WB (1:1000) |
| Antibody | Anti-MAVS (mouse polyclonal) | Cell Signaling Technology | Cat# 3993 | WB (1:1000) |
| Antibody | Anti-DCAF1(rabbit polyclonal) | Bethyl | Cat# A301-887A | WB (1:1000) |
| Antibody | Anti-Nup358 (rabbit polyclonal) | Abcam | Cat# ab64276 | WB (1:1000) |
| Antibody | Anti-FLAG (mouse monoclonal) | Sigma | Cat# F3165 | WB (1:1000) |
| Antibody | Anti-GFP (rabbit polyclonal) | Abcam | Cat# ab6556 | WB (1:20,000) |
| Antibody | Anti-KPNA1 (rabbit polyclonal) | ABclonal | Cat# A1742 | WB (1:1000) |
| Antibody | Anti-KPNA2 (rabbit polyclonal) | ABclonal | Cat# A1623 | WB (1:1000) |
| Antibody | Anti-KPNA3 (rabbit polyclonal) | ABclonal | Cat# A8347 | WB (1:1000) |
| Antibody | Anti-KPNA4 (rabbit polyclonal) | ABclonal | Cat# A2026 | WB (1:1000) |
| Antibody | Anti-KPNA5 (rabbit polyclonal) | ABclonal | Cat# A7331 | WB (1:1000) |
| Antibody | Anti-KPNA6 (rabbit polyclonal) | ABclonal | Cat# A7363 | WB (1:1000) |
| Antibody | Anti-KPNB1 (rabbit polyclonal) | ABclonal | Cat# A8610 | WB (1:1000) |
| Antibody | Anti-CypB (rabbit polyclonal) | Abcam | Cat# ab16045 | WB (1:5000) |
| Antibody | Anti-HA (rabbit polyclonal) | Sigma | Cat# H6908 | WB (1:1000) |
| Antibody | Anti-Vpr (rabbit polyclonal) | NIH AIDS reagents programme | Cat# 11836 | WB (1:1000) |
| Antibody | Anti-mouse IgG IRdye 800CW (goat poly clonal) | LI-COR Biosciences | Cat# 926–32210 | WB (1:10,000) |
| Antibody | Anti-rabbit IgG IRdye 800CW (goat poly clonal) | LI-COR Biosciences | Cat# 926–32211 | WB (1:10,000) |
| Other | Herring testes DNA | Sigma | Cat# D6898 | Amount used stated in text |
| Other | cGAMP | Invivogen | Cat code (tlrl-nacga23-1) | Amount used stated in text |
| Other | Poly I:C | Invivogen | Cat code (tlrl-pic) | Amount used stated in text |
| Other | Lipopolysaccaride | Invivogen | Cat code (tlrl-smlps) | Amount used stated in text |