Table 2.
Primers used in the study.
| Items | Gene | Sequence (5′-3′) |
|---|---|---|
| BSH gene | BSH1 | F: GCCACCATGGTAATGTGCACGGCCGTTTCC |
| R: CGATGGATCCTTAGGGTACTTGCGATAGG | ||
| BSH2 | F:ACCCATGGGTATGTGCACGAGCATCAACGTCA | |
| R: AAGGATCCGTTCAATTTCACCGGCGCCCAA | ||
| BA receptor and signaling | FXR | F: GGTCCTCGTAGAATTCACAA |
| R: TGAACGGAGAAACATAGCTT | ||
| FGF19 | AGTACTCGGATGAGGACTGTGCTT | |
| AGAGACGGGCAGATGGTGTTTCTT | ||
| SHP | F: GCCTACCTGAAAGGGACCAT | |
| R: CAACGGGTGTCAAGCCTTTA | ||
| BA transport | ASBT | F: TACGCGGTATACAGGAAATGGTA |
| R: TTTGCCTTTTGGAATGATGACT | ||
| IBABP | F: GTGAACAGCCCCAACTACCACCA | |
| R: TCGTAGCTCACGCCTCCGAC | ||
| BA biosynthesis | CYP7A1 | F: GAAAGAGAGACCACATCTCGG |
| R: GAATGGTGTTGGCTTGCGAT | ||
| CYP27A1 | F: ACTGAAGACCGCGATGAAAC | |
| R: CAAAGGCGAATCAGGAAGGG | ||
| Cholesterol biosynthesis and transport | SREBP-2 | F: GATGGGCAGCAGAGTTCC |
| R: ACAGCAGCAGGTCACAGGT | ||
| HMGR | F: ATGGCATGACTCCAGTGGTACGTT | |
| R: GCAAATCTGCTGGTGCTGTCGAAT | ||
| HDLR | F: CACTATGCCCAGTACGTGCTC | |
| R: CCTGAATGGCCTCCTTATCCTT | ||
| LDLR | F: TTCTTCACCAACCGCCACGAG | |
| R: CTCAGTGTCCAGAGCGACC | ||
| Housekeeping gene | GAPDH | F: ATGGTGAAGGTCGGAGTGAAC |
| R: CTCGCTCCTGGAAGATGGT |
BSH, bile salt hydrolase; FXR, farnesoid X receptor; FGF19, fibroblast growth factor; SHP, small heterodimer partner; ASBT, apical sodium-dependent bile acid transporter; IBABP, ileal bile acid binding protein; CYP7A1, Cholesterol-7α hydroxylase; CYP27A1, cholesterol-27α hydroxylase; SREBP-2, sterol regulatory element binding protein-2; HMGR, 3-hydroxy-3-methyl glutaryl coenzyme A reductase; HDLR, high-density lipoprotein receptor; LDLR, low-density lipoprotein receptor.