Skip to main content
. 2020 Dec 11;11:608314. doi: 10.3389/fmicb.2020.608314

FIGURE 4.

FIGURE 4

Safety traits of Enterococcus durans OSY-EGY. (A) Gelatinase activity (Left panel) of E. durans OSY-EGY compared to Bacillus cereus ATCC 14579 (positive control) and Escherichia coli k12 (negative control) on nutrient agar containing 3% gelatin and flooded with ammonium sulfate saturated solution. Clear zone indicates gelatin hydrolysis due to gelatinase production. Hemolytic activities (right panel) of E. durans OSY-EGY on blood agar compared to S. aureus ATCC 25923 as positive control and E. faecalis ATCC 29212 as negative control. Clear zone indicates red blood cells lysis due to hemolysin production. (B) Cytotoxic effect of E. durans OSY-EGY or E. Faecalis (Symbioflor 1) strains on Caco-2 cells after 24 h treatments. Values were expressed as means ± SD of two independent repeats. Treatments that were significantly different at P ≤ 0.05 were indicated by different letters. (C) Microscopy observation of Caco-2 cells after 3 h of treatment with E. durans OSY-EGY or E. faecalis Symbioflor 1 compared to untreated Caco-2 cells.