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. 2020 Dec 11;11:596308. doi: 10.3389/fgene.2020.596308

FIGURE 5.

FIGURE 5

Sanger sequencing result of 328-177, 294-177, and 34-434. (A) Sanger sequencing of 328-177 and 294-177 confirmed the back-splicing junction in PCR products. Some overlapping peaks might mean that more than two transcripts formed by alternative splicing. (B) Sanger sequencing of 34-434 confirmed the back-splicing junction in PCR products.