Figure 2.

Islet morphology and beta-cell function. (A) Immunofluorescent images in the islets. A white bar indicates 50 µm. Insulin: green, glucagon: red, DAPI: blue. x200 magnification. (B) Beta-cell/alpha cell area ratio. From three mice, a of total 34–57 islets were counted. Data are presented as means ± SEM. n=6, each group. *p < 0.05, **p < 0.01, ****p < 0.0001. (C) Glycemic change during oral glucose load. (D) Glucose-stimulated insulin secretion by 2 g/Kg body weight oral glucose load. Delta insulin levels (insulin at 15 min minus insulin at 0 min) are shown. White bars, normal-carbohydrate diet (NC); hatched white bars, low-carbohydrate diet (LC); grey bars, NC+Ipra; hatched gray bars, LC+Ipra (B, D). White squares, NC; black squares, LC; white circles, NC+Ipra; black circles, LC+Ipra (C).