Figure 2.
MgCl2 affected apoptosis and ER stress in bladder cancer cells UC3. (A) Expression of apoptosis-related genes as determined by qRT-PCR. (B) ER stress-related gene expression as revealed via qRT-PCR. (C) Protein expression of apoptosis-related genes as revealed via western analysis. (D) Protein expression of ER stress-related genes as determined by Western blot analysis. Data were expressed as mean ± SEM of duplicate experiments. The differences between the two groups were assessed using the unpaired Student’s t-test. *p < 0.05 versus control, and **p < 0.01 versus control.