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. 2020 Dec 11;10:589112. doi: 10.3389/fonc.2020.589112

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Copyright © 2020 Li, Yu, Shi, Cao, Liu, Hao, Ren, Qin, Huang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MgCl₂ affected the migration of UC3 cells. (A) Protein expression of EMT related genes as evaluated by Western blot analysis. (B) mRNA expression of cancer stem cell marker genes was determined by qRT-PCR in the cells treated with MgCl₂ for 24 h. (C) mRNA expression of cancer stem cell marker genes was determined by qRT-PCR in the cells treated with MgCl₂ for 48 h. Data were expressed as mean ± SEM of duplicate experiments. The differences between the two groups were assessed using the unpaired Student’s t-test. *p < 0.05 versus control.