Figure 8.

Combination treatment with MgCl₂ and VPA affected cell survival, expression of genes related to cell cycle progression, and in vivo tumorigenicity of UC3 cells. (A) The survival rate of cells with combination treatment as determined by CCK-8 assay. (B–F) mRNA expression of genes related to cell cycle progression was investigated by qRT-PCR. Data were expressed as mean ± SEM of duplicate experiments. (G) Western blot analysis of genes related to cell cycle progression. (H) UC3 cells were injected into BALB/c nude mice, as indicated. After therapy with MgCl₂ and/or VPA for 14 days, mice were sacrificed to harvest tumors. The tumor volume (I) and weight (J) were inhibited by combination treatment compared with the control group. The differences among multiple groups were assessed using the Analysis of variance (ANOVA). *p < 0.05 versus control, **p < 0.01 versus control, and ***p < 0.001 versus control.