Figure 7.

Impact of IFN-γ blockade. Egg-allergic mice were treated daily on three consecutive days with IL-2/JES6 and challenged with allergen after the last IL-2/JES6 injection. Some groups additionally received one or three injections of anti-IFN-γ blocking antibodies, with all or only the last injection of IL-2/JES6, respectively. Mice were challenged 4 h after the last IL-2/JES6 injection. (A) Temperature drop curve. Representative data from one of two experiments are shown (B) Changes of maximal temperature drops before and after anti-IFN-γ blockade in individual mice (left, statistics: two way ANOVA with Sidak’s multiple comparison test) and maximal temperature drops after treatment in the various groups (right, statistics: Kruskal-Wallis Test with Dunn’s multiple comparison test) are shown, as indicated. Data are pooled from two independent experiments. (C) Blood was drawn 1.5 h after the challenge and serum levels of mMCP-1 and mMCP-7 were measured by ELISA. Data represent mean ± SEM. Representative data from one of two experiments are shown (statistics: Kuskal-Wallis Test with Dunn’s multiple comparison test). *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001.