FIGURE 3.

p38 MAPK inhibition attenuates I/R injury-induced damage to endothelial integrity. (A) Western blot of phospho-p38 MAPK, p38 MAPK, AQP1, ICAM-1, ZO-1, and VE-cadherin in lung tissues. (B–F) Densitometry of western blots from panel A. Levels were normalized to the control, which was defined as 1.0. (G,I) Immunohistochemistry of ZO-1 and VE-cadherin in lung tissues. Normal rabbit IgG in Phosphate-buffered saline (PBS) was used in the immunohistochemistry experiment instead of the primary antibody as a negative control. Rat spleen and kidney tissue instead of lung tissue were used for ZO-1 and VE-cadherin immunohistochemistry, respectively, as a positive control. Control group mice were treated without vehicle, drug, or surgery. Scale bar: 200 μm. (H,J) Quantification of ZO-1 and VE-cadherin immunohistochemical staining. n = 6 tissue sections per group. ns, not significant, *p < 0.05.