Table 1.
Details of primers used for the qPCR verification study.
| Gene | Primer sequence | PCR efficiency (%) | Amplicon size (bp) | GenBank accession numbers |
|---|---|---|---|---|
| anxa2 | CATTGCAGAAAGAATACAAAGGGG-F | 92.1 | 96 | XM_014161401.1 |
| CCAGCGTGACAATACTGTG-R | ||||
| cath1b | GTCCTCTGAAGAAAAATGGGAAAC-F | 88.5 | 135 | NM_001123586.1 |
| GCATAGCATCTTCTGCCTC-R | ||||
| cath2 | CCGATTCTGGAGACTGGCAA-F | 96.5 | 111 | NM_001123573.1 |
| TGTCCGAATCTTCTGAGTGC-R | ||||
| clcn1 | TCAGCAACAACAGTCTCT-F | 93.0 | 82 | XM_014152555.1 |
| GCTGTGGATGGTGCTGTT-R | ||||
| clcn2 | CTCGGACACATCAGTAAG-F | 90.8 | 123 | XM_014184291.1 |
| TGAGGGAGGTGGAGTCTAGC-R | ||||
| csad | CGGTCTGGCTGACATAAT-F | 88.2 | 127 | NC_027317.1 |
| AGTTGACTCGTCCACCCTGA-R | ||||
| gal3 | CGGAGCTACTAACAGATA-F | 90.1 | 127 | NM_001140833.1 |
| GTTGGCTGGTTGGGTTGC-R | ||||
| gsto1 | GCTTCATGCCAAGGGGAT-F | 89.9 | 107 | NM_001141472.1 |
| TCTCCAATGTCGGAACCAGG-R | ||||
| lysc2 | ATGAGAGCTGTTGTTGTTC-F | 97.0 | 144 | XM_014145497.1 |
| AGACAGGCACACCCAGTT-R | ||||
| mta | TGAATGCTCCAAAACTGG-F | 88.3 | 130 | NM_001123677.1 |
| CCTGAGGCACACTTGCTG-R | ||||
| rbp2 | GACCTGCTACACCTGGACATC-F | 104.0 | 147 | NM_001146482.1 |
| TCTCAACTGGCCTACCTG-R | ||||
| slc26a6 | TGGGCATGGAACACCTGA-F | 91.1 | 124 | NC_027312.1 |
| CACCAACTGTTAAACTCG-R | ||||
| slc6a19 | ATGGAGGAGGAGCGTTTA-F | 100.5 | 158 | NM_001141815.1 |
| CGATGCCAACACCTGTCAGA-R | ||||
| slc6a6 | GGTGTAATTCATTTCCGATGC-F | 95.8 | 109 | XM_014134772.1 |
| CTCTTTCTGTGCCATGCTGC-R | ||||
| tnfrsf1b | TCGGAGGTGTTATCGGAG-F | 91.7 | 80 | XM_014133111.1 |
| CCTGGACCCTGTGAAGACTTT-R | ||||
| Reference genes | ||||
| ef1ab | TGCCCCTCCAGGATGTCTAC-F | 94.5 | 59 | BG933853 |
| CACGGCCCACAGGTACTG-R | ||||
| rpl13 | CGCTCCAAGCTCATCCTCTTCCC-F | 95.5 | 79 | BT048949.1 |
| CCATCTTGAGTTCCTCCTCAGTGC- R | ||||
| rps29 | GGGTCATCAGCAGCTCTATTGG-F | 93.3 | 167 | BT043522.1 |
| AGTCCAGCTTAACAAAGCCGATG-R | ||||
| ubi | AGCTGGCCCAGAAGTACAACTGTG-F | 94.9 | 162 | AB036060.1 |
| CCACAAAAAGCACCAAGCCAAC-R | ||||
Annexin 2-like (anxa2), cathelicidin 1-B (cath1b), cathelicidin 2 (cath2), chloride channel protein 1-like (clcn1), chloride channel protein 2-like (clcn2), cysteine sulfinic acid decarboxylase-like (csad), galectin-3 (gal3), glutathione S-transferase omega-1(gsto1), lysozyme C II (lysc2), metallothionein A (mta), retinol binding protein II (rbp2), solute carrier family 26 member 6-like (slc26a6), solute carrier family 6 member 19 (slc6a19), solute carrier family 6 (neurotransmitter transporter, taurine) member 6 (slc6a6), and tumor necrosis factor receptor superfamily member 1B-like (tnfrsf1b).