Table 2.

Kinetic Parameters for Trypsin Proteolysis.^a

Peptide	k1 (μM−1•min−1)	k−1 (min−1)	k2 (min−1)
Lys Controlb	0.49 ± 0.03	<2.8 × 10−6	106.2 ± 0.9
Lys P1b	0.41 ± 0.01	46.6 ± 0.8	1.31 ± 0.01
Arg Controlb	5.72 ± 0.11	<8.4 × 10−5	273.6 ± 3.1
Arg P1b	0.44 ± 0.16	2.88 ± 0.96	0.126 ± 0.002

^aCleavage measurements (Figs. S6 and S7) and global fitting data analysis as described in SI.

^bSequences: Lys Control (μLLKAAAμ), Lys P1 (μLLK^SAAAμ), Arg Control μLLRAAAμ), Arg P1 (μLLR^SAAAμ).