Table 4.
Effects of partially replacing maize silage and barley straw in a mixed diet (CON) by crude olive cake (COC) on microbial abundance and bacterial diversity in solid and liquid phases of Rusitec fermenters (n = 4).
| Digesta Phase | Item 1 | Diet | SEM | p-Value | |
|---|---|---|---|---|---|
| CON | COC | ||||
| Solid | Total bacteria 2 | 101 | 193 | 12.2 | 0.01 |
| Total protozoa 2 | 0.0015 | 0.0067 | 0.00106 | 0.06 | |
| Fungi 3 | 46.7 | 36.6 | 19.33 | 0.80 | |
| Archaea 2 | 0.03 | 0.05 | 0.006 | 0.36 | |
| Number of peaks | 21.0 | 22.5 | 0.85 | 0.56 | |
| Shannon index | 3.04 | 3.09 | 0.029 | 0.66 | |
| Liquid | Total bacteria 2 | 1.75 | 2.01 | 0.246 | 0.72 |
| Total protozoa 2 | 0.0002 | 0.0002 | 0.00002 | 0.99 | |
| Fungi 3 | 0.006 | 0.015 | 0.0022 | 0.25 | |
| Archaea 2 | 0.02 | 0.39 | 0.156 | 0.17 | |
| Number of peaks | 29.2 | 30.2 | 1.06 | 0.81 | |
| Shannon index | 3.37 | 3.39 | 0.093 | 0.85 | |
1 Abundance of bacteria and protozoa DNA and the relative abundance of fungi and archaea were determined by qPCR, and bacterial diversity was analyzed by automated ribosomal intergenic spacer analysis (ARISA); 2 Expressed as µg DNA/g dry matter in the solid phase and as µg DNA/mL in the liquid phase; 3 Expressed as relative abundance to the absolute quantification of total bacteria as 2―(CT target―CT total bacteria).