Table 1.
Forward and reverse primers of the genes analyzed using ddPCR.
| NCBI Accession | Gene ID | Sequence (5’→3’) | Amplicon Size (bp) | Template 1 (ng) |
|---|---|---|---|---|
| XR_001466725.2 | HIF-1A | F: ATCAGAGTGGTTGTCCAGCAG R: CAGTCCAAGCCCACCTTACT |
111 | 25 |
| NM_205284.1 | LDHA | F: TTCTCTGCCAGCTGAATAGCTT R: CGGGTCATTGTCTTGTTGCAT |
200 | 1 |
| NM_001031352.3 | PDK1 | F: TGCCAAGCAGTGAGCCAAAG R: AACTCCCTTCACATGACACACAT |
97 | 10 |
| XM_015285700.1 | SOD3 | F: TACAAACCCAACCTCTTCGC R: GTTATTGCCCTTGCCCATGT |
102 | 10 |
1 cDNA template (ng) in 20 μL ddPCR reaction, obtained from preliminary optimization. HIF-1A = hypoxia-inducible factor 1 alpha subunit, LDHA = lactate dehydrogenase A, PDK1 = pyruvate dehydrogenase kinase isozyme 1, SOD3 = superoxide dismutase 3.