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. 2020 Dec 1;9(12):2574. doi: 10.3390/cells9122574

Figure 4.

Figure 4

Spontaneous activity of ON- and OFF-SACs during glutamatergic waves. ON- and OFF-SACs from flat-mount retinas of ChAT-Cre/tdTomato mice were recorded from P8 to P14 using a whole-cell current-clamp configuration. (A) An ON-SAC exhibited spontaneous rhythmic depolarization, and most of the depolarization had two peaks at P9 (top trace). An insert illustrates that the APs were superimposed onto the initial (#) and delayed peaks (##) on an extended time scale. CNQX (50 µM) and D-AP5 (50 µM) completely blocked the spontaneous activity (bottom trace). (B) An OFF-SAC also exhibited spontaneous rhythmic depolarization at P9, and the depolarization had initial (#) and delayed (##) peaks (see the insert with an extended time scale). CNQX (50 µM) and D-AP5 (50 µM) completely blocked the spontaneous activity (bottom trace). (C) The ON-SACs were pooled into two groups: P8–P9 and P10–P14. The bars show the percentages of S-spiking (black), non-S/C-spiking (gray), and non-S/non-C-spiking (black dots) ON- and OFF- SACs. (D) The ON- and OFF-SACs recorded from P8–P14 were pooled. The bars illustrate the percentages of S-spiking (black), non-S/C-spiking (gray), and non-S/non-C-spiking ON-SACs (block dots) for each SAC subtype. The number of SACs tested is presented within the bars for (C,D).