Table 2.

Summary of DNA sequencing and IHC of H3K36me3.

Case	Gene	Mutation Type	CNV	Exon	AA Changes	SIFT Prediction	H3K36me3 IHC	COSMIC ID
1	SETD2	Inflame indel	NA	exon 20	p.Phe2481-Gln2484del	Probably damaging	Negative	None
	JAK3	Missense	NA	exon 13	p.Ala573Val	Damaging	-	COSM34215(17)
2	SETD2	Frameshift indel	Normal	exon 20	p.Tyr2489Trpfs*6	Probably damaging	Negative	None
	JAK1	Missense	Normal	exon 22	p.Lys1026Glu	Probably damaging	-	None
3	SETD2	Missense	Normal	exon 7	p.Cys1631Phe	Incertain	Negative	None
	JAK3	Missense	Normal	exon 13	p.Ala573Val	Damaging	-	COSM34215(17)
	STAT5B	Missense	Normal	exon 18	p.Val712Glu	Damaging	-	COSM5414165(1)
4	SETD2	Missense	Loss	exon 20	p.Leu2486Arg	Probably damaging	Negative	None
	JAK1	Missense	Normal	exon 15	p.Ser703Ile	Damaging	-	COSM305942(10)
	TP53	Missense	Normal	exon 7	p.Gly244Asp	Damaging	-	COSM10735(43)
5	SETD2	Substitution (intron)	Normal	exon 5	-	Probably damaging	Negative	None
	JAK3	Splicing	Normal	exon 20	-	Incertain	-	None
		Missense		exon 13	p.Ala573Val	Damaging	-	None
	STAT5B	Missense	Normal	exon 16	p.Asn642His	Damaging	-	COSM1716590(29)
6	SETD2	WT	Loss	-	-	-	Positive	None
	JAK3	Missense	Normal	exon 11	p.Met511Ile	Probably damaging	-	COSM51374(29)
7	SETD2	Splicing	NA	exon 1	-	Probably damaging	Negative	None
		Splicing		exon 6	-	Probably damaging		None
	JAK1	Missense	NA	exon 17	p.Leu783Phe	Probably damaging	-	COSM41758(5)
	JAK3	Missense	NA	exon 15	p.Val674Ala	Probably damaging	-	COSM327317(7)
8	SETD2	WT	Loss	-	-	-	Negative	None
	JAK1	Missense	Normal	exon 15	p.Ser703Cys	Incertain	-	None
	JAK3	Missense	Normal	exon 15	p.Val674Ala	Probably damaging	-	COSM327317(7)
	PIK3CD	Missense	Normal	exon 8	p.Met339Lys	Incertain	-	None
	TP53	Missense	Normal	exon 10	p.Arg337His	Probably damaging	-	None
9	SETD2	Missense	Loss	exon 11	p.Ser1769Tyr	Incertain	Negative	None
	STAT5B	Missense	Normal	exon 16	p.Asn642His	Damaging	-	COSM1716590(29)
	ATM	Missense	Normal	exon 19	p.Pro960His	Incertain	-	None

The data of CNV are from reference [15]. Abbreviations: CNV, copy number variation; COSMIC, Catalogue of Somatic Mutation in Cancer; NA, not analysed.