Table 3.

Sequences, relative intensity (RI), and predicted emulsifying activity (score) in α-helical, β-strand, and γ (axial amphiphilicity) conformation for the 20 peptides with highest relative intensity (PCL > 6) from enzymatically derived FPH from minced cod frame (MCF) and heated cod meat (HCM). Emulsifier prediction scores > 2 (topmost 2.5%) are highlighted in bold.

MCF
Neut.	RI (%)	Emulsifying Scores			Alc	RI (%)	Emulsifying Scores			Neut&Alc	RI (%)	Emulsifying Scores
		α	β	γ			α	β	γ			α	β	γ
LQGEVEDLMVDVERANG	1.94	1.38	−0.45	0.55	GFAGDDAPRAVFPS	2.30	0.29	−0.86	0.71	IIAPPERKYS	3.49	−0.59	−0.62	3.43
LEQQVDDLEGSLEQEKK	1.92	2.18	−0.87	1.26	RVAPEEHPTLL	2.13	0.36	−0.09	1.79	GVDNPGHPF	2.00	1.13	−0.49	0.08
IITNWDDMEK	1.78	0.75	−1.28	2.95	AGDDAPRAVFPS	2.09	0.97	−1.33	1.23	VAPEEHPTL	1.59	−0.18	−0.07	1.04
VQHELEEAEERADIAETQVNK	1.39	2.21	−0.41	0.64	AGPAGPSGPRGPAGIA	1.56	0.16	−0.17	0.47	VLYPPVVEE	1.53	−0.43	−1.16	0.55
LTKLEEAEKAADESERGMK	1.17	1.53	−0.55	1.15	KSYELPDGQVITIG	1.33	−0.78	−0.79	2.64	GADPEDVIVA	1.43	−1.29	−1.00	3.10
LEDQLSEIKAKSDENARQ	1.00	1.54	0.10	1.33	GAAGPAGPSGPRGPAGIA	1.30	0.16	−0.51	0.23	VIDQDKSGFIE	1.35	0.37	−0.43	0.88
LEKSYELPDGQVIT	0.92	−0.55	−0.43	0.82	DIDIRKDLYAN	1.16	−1.24	3.65	0.83	AGDDAPRAVFPS	1.20	0.97	−1.33	1.23
VAPEEHPTL	0.90	−0.18	−0.07	1.04	VAPEEHPTLL	1.12	−0.53	−0.81	1.14	KSYELPDGQ	1.19	−1.09	−0.94	−0.55
IIDQNRDGIISKDDLRD	0.86	−0.51	−0.56	0.70	AGPSGPRGPAGIA	1.08	−0.23	−0.12	1.26	GERGEQGPGGPGGF	1.08	−0.79	−0.73	1.36
LDDLQAEEDKVNT	0.81	0.55	−1.02	1.17	NWDDMEKIWHH	1.06	0.75	−0.27	1.02	IIDQNRDGIIS	0.83	0.68	−0.86	1.67
LEKTIDDLEDELYAQK	0.78	1.51	−0.63	0.12	GQKDSYVGDEAQSKRGILTL	0.92	−0.71	−0.30	1.27	AGPAGPSGPRGPAG	0.81	−0.56	−0.48	0.21
LKGTEDELDKYSEALKDAQEKLE	0.77	2.96	0.22	0.47	LRVAPEEHPTL	0.88	0.64	1.15	1.24	GLPGPSGPSGPRGR	0.78	−0.81	−1.11	0.80
LTEEMASQDESVAK	0.76	1.01	−0.17	0.11	RGDSGPAGPPGEQGML	0.84	−1.16	−0.62	0.13	RGEQGPGGPGGF	0.76	−1.42	−0.18	1.04
LKGADPEDVIVAA	0.76	−1.53	−0.19	2.78	ELPDGQVITIG	0.82	−0.99	0.01	2.07	GFAGDDAPRA	0.69	0.45	−0.67	1.06
LADWKQKYEEGQAELEGSLKEARS	0.71	0.70	−0.08	0.43	SGSAGKDGMSGLPGPSGPSGPRGR	0.67	−0.23	−1.12	0.32	AINDPFIDL	0.67	0.83	−0.47	0.47
SKYETDAIQRTEELEESKKK	0.69	0.71	−0.69	1.57	LLPVLYPPVVEE	0.67	0.24	−1.23	1.33	KAGDSDGDGAIGVD	0.59	−0.30	0.65	1.81
LKAGDSDGDGAIGVDEWAV	0.69	0.26	−0.72	1.09	SGPGGPTGPSGM	0.64	−1.24	−0.74	−0.49	GFAGDDAPRAVFPS	0.58	0.29	−0.86	0.71
LTDAETKAF	0.65	0.43	−0.14	−0.44	KSYELPDGQVITIGNE	0.62	−0.17	−0.82	1.35	LEDQLSELK	0.54	1.36	−1.20	−0.22
LKAGDSDGDGAIGVDEWAVLVKA	0.61	−0.98	−1.16	1.53	GPAGPSGPRGPAGIA	0.59	−0.10	−0.37	0.91	KILDPEAT	0.52	0.59	−0.71	0.96
VDDIIQTGVDNPGHPFIMT	0.59	0.89	0.34	0.07	FAGDDAPRAVFPS	0.58	0.35	−0.89	0.70	GKDGMSGLPGPSGPSGPRGR	0.52	−0.08	−0.72	0.47
HCM
Neut.	RI (%)	α	β	γ	Alc	RI (%)	α	β	γ	Neut&Alc	RI (%)	α	β	γ
LEQQVDDLEGSLEQEKK	2.38	2.18	−0.87	1.26	AGDDAPRAVFPS	3.13	0.97	−1.33	1.23	VAPEEHPTL	1.91	−0.18	−0.07	1.04
LQGEVEDLMVDVERANG	2.28	1.38	−0.45	0.55	GPAGPSGPRGPAGIA	2.64	−0.10	−0.37	0.91	VLYPPVVEE	1.75	−0.43	−1.16	0.55
IITNWDDMEK	2.23	0.75	−1.28	2.95	AGPAGPSGPRGPAGIA	1.90	0.16	−0.17	0.47	IIDQNRDGIIS	1.45	0.68	−0.86	1.67
VQHELEEAEERADIAETQVNK	1.46	2.21	−0.41	0.64	VAPEEHPTLL	1.87	−0.53	−0.81	1.14	ELPDGQVIT	1.14	−1.76	−0.72	1.69
LEKSYELPDGQVIT	1.27	−0.55	−0.43	0.82	GFAGDDAPRAVFPS	1.75	0.29	−0.86	0.71	VIDQDKSGFIE	1.13	0.37	−0.43	0.88
VETEKTEIQSALEEAEGTLEHEESKILR	1.00	2.29	−0.80	0.25	GAAGPAGPSGPRGPAGIA	1.62	0.16	−0.51	0.23	IIAPPERKYS	1.11	−0.59	−0.62	3.43
LDDLQAEEDKVNT	0.94	0.55	−1.02	1.17	RVAPEEHPTLL	1.53	0.36	−0.09	1.79	LEDQLSELK	1.06	1.36	−1.20	−0.22
LADWKQKYEEGQAELEGSLKEARS	0.74	0.70	−0.08	0.43	ELPDGQVITIG	1.34	−0.99	0.01	2.07	GVDNPGHPF	1.00	1.13	−0.49	0.08
VAPEEHPTL	0.72	-0.18	−0.07	1.04	KSYELPDGQVITIG	1.21	−0.78	−0.79	2.64	GADPEDVIVA	0.95	−1.29	−1.00	3.10
LDDVIQTGVDNPGHPFIMT	0.71	0.83	0.33	−0.02	LLPVLYPPVVEE	1.09	0.24	−1.23	1.33	AAGPAGPSGPRGPAG	0.75	−0.56	−0.75	0.55
LEDECSELKKDIDDLELT	0.71	1.84	−0.42	0.82	LGEQIDNL	1.03	2.57	−0.40	−0.40	AGDDAPRAVFPS	0.71	0.97	−1.33	1.23
ARIEELEEELEAERA	0.68	−0.65	−0.93	0.54	RGDSGPAGPPGEQGML	0.94	−1.16	−0.62	0.13	LDKNKDPLNDSVVQ	0.68	1.41	−0.79	1.18
LTEEMASQDESVAK	0.67	1.01	−0.17	0.11	GSAGPRGPSGNIGMPGMTGPQ	0.85	−1.75	−0.73	0.14	FAGDDAPRA	0.67	0.49	−0.69	1.15
IEELEEELEAERA	0.65	0.22	0.05	1.05	TIIDQNRDGIIS	0.76	0.70	−0.99	1.49	ELPDGQVITI	0.67	−0.93	0.00	2.37
LDFENEMAT	0.65	0.10	1.04	0.59	GEKLKGADPEDVIVA	0.74	−1.01	−0.41	2.36	KSYELPDGQVITIG	0.63	−0.78	−0.79	2.64
VDDIIQTGVDNPGHPFIMT	0.63	0.89	0.34	0.07	SGSAGKDGMSGLPGPSGPSGPRGR	0.67	−0.23	−1.12	0.32	GFNPPDLDIM	0.60	−0.75	−0.19	1.20
LKAGDSDGDGAIGVDEWA	0.61	0.26	−0.18	0.56	SGPGGPTGPSGM	0.67	−1.24	−0.74	−0.49	ELPDGQVI	0.60	−1.92	−0.85	0.68
LVQVQGEVDDSVQEARNAEEKAKKA	0.60	2.64	1.54	2.42	GEQIDNL	0.67	1.96	−1.14	0.44	NPPKYDKIEDM	0.55	−0.15	−0.70	1.79
ALEEAEGTLEHEESKLLR	0.58	1.83	−0.16	0.57	ELPDGQVI	0.66	−1.92	−0.85	0.68	KSYELPDGQVITI	0.53	−0.70	−0.82	2.76
IEELEEELEAERAAR	0.56	−0.70	0.82	0.31	KSYELPDGQVITI	0.65	−0.70	−0.82	2.76	KSYELPDGQVIT	0.51	−1.28	−1.17	1.13