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. 2020 Nov 30;12(12):3713. doi: 10.3390/nu12123713

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of WEST pretreatment on the expression of apoptosis-related proteins in corticosterone-treated PC12 cells. Protein expression of (A) Bax, (B) Bcl-2, (C) cytochrome c, (D) caspase-9, and (E) caspase-3 in PC12 cells pretreated with WEST and treated with corticosterone compared to corticosterone-treated and non-treated cells. The protein levels were normalized to the GAPDH protein level. (F) Western blot image of Bax, Bcl-2, cytochrome c, caspase-9, caspase-3, and GAPDH protein expression. CON, non-treated control; CORT, 200 µM corticosterone treatment; WEST+CORT, 50 µg/mL WEST pretreatment plus 200 µM corticosterone treatment. Bars represent mean ± SD. ** p < 0.01 compared with CON group; * p < 0.05 compared with CON group; ## p < 0.01 compared with CORT group; # p < 0.05 compared with CORT group. kDa; kilodalton.