Figure 1.

Antimicrobial Peptides (AMPs) discovery framework. Rational design steps: (I) Deep learning techniques identify sequences with potential antimicrobial activity, (II) membrane-disruption capabilities of selected sequences are analyzed via molecular dynamics (MD), (III) the host cell is modified, and sequences are inserted, finally (IV) antimicrobial activity is corroborated by a microfluidic system. Non-rational design steps: (I) Random sequences are expressed on host cells through cell surface display, (II) modified microorganisms are analyzed by a microfluidics system to obtain AMPs candidates, and (III) DNA is extracted, sequenced, and cloned (Created with BioRender).