Figure 1.

ATP assay of chondrocytes cultured in monolayers after treatment with 0 mg/mL, 10 mg/mL, 20 mg/mL and 50 mg/mL of tranexamic acid for varying exposure times. Chondrocytes from five different donors were seeded in cell culture flasks, incubated in cell culture medium and grown to confluency. After reaching confluency, cells were exposed to 0 mg/mL (no TXA), 10 mg/mL, 20 mg/mL or 50 mg/mL of TXA. The exposure time varied from 10 min to 24 h to 48 h. Chondrocyte proliferation was evaluated using the ATP assay. Each dot represents a single value from all five examined donor samples after treatment with different concentrations of TXA for indicated exposure times. * Significant difference (p < 0.05) compared to control samples. TXA, tranexamic acid; min, minutes; d, days; h, hours.