Figure 2.

ATP assay of mesenchymal stromal cells following osteogenic differentiation and treatment with 50 mg/mL tranexamic acid for varying exposure times. Osteogenic differentiation was induced in primary hMSCs harvested from five different donors using an osteogenic differentiation medium for 21 d. Undifferentiated control samples (undiff.) were maintained for comparison. After 21 d of osteogenesis, MSCs were exposed to 0 mg/mL (no TXA), 10 mg/mL, 20 mg/mL or 50 mg/m of TXA. Cells were exposed to TXA for 10 min, 24 h or 48 h. Cell proliferation was evaluated using the ATP assay. Each dot represents a single value from all five examined donor samples after treatment with different concentrations of TXA for indicated exposure times. * Significant difference (p < 0.05) compared to control samples. hMSCs, human mesenchymal stromal cells; TXA, tranexamic acid; min, minutes; d, days; h, hours.