Figure 6.

Relative changes in the expression of osteogenic marker genes pictured as dot plots as measured by semiquantitative RT-PCR in osteogenic differentiated mesenchymal stromal cells after treatment with 0 mg/mL, 10 mg/mL, 20 mg/mL and 50 mg/mL of tranexamic acid for varying exposure times. hMSCs were derived from bone marrow reamings of five patients that underwent total hip arthroplasty. Cells were seeded in cell culture flasks and incubated in osteogenic differentiation medium for 21 d. Undifferentiated cells (undiff.) were maintained for comparison. Following osteogenesis cells were exposed to 0 mg/mL (no TXA), 10 mg/mL, 20 mg/mL or 50 mg/mL of TXA. The exposure time varied from 10 min to 24 h to 48 h. Each dot represents changes in the relative expression of osteogenic marker genes collagen type I alpha 1 chain (COL1A1), collagen type X alpha 1 chain (COLXA1), alkaline phosphatase (ALP) and osteocalcin (OC) in a single donor sample in dependance of the TXA concentrations (a–c) and exposure times (d–f) are pictured, respectively. Eukaryotic elongation factor 1α (EEF1A1) was used as the housekeeping gene and for internal controls. Primer details are illustrated in Table 1. d, days; hMSCs, human mesenchymal stromal cells; TXA, tranexamic acid, h, hours; min, minutes.